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| [[Image:1kk2.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1kk2| PDB=1kk2 | SCENE= }} | | {{STRUCTURE_1kk2| PDB=1kk2 | SCENE= }} |
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| '''Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant complexed with GDP-Mg2+'''
| | ===Structure of the large gamma subunit of initiation factor eIF2 from Pyrococcus abyssi-G235D mutant complexed with GDP-Mg2+=== |
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| ==Overview==
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| The heterotrimeric factor e/aIF2 plays a central role in eukaryotic/archaeal initiation of translation. By delivering the initiator methionyl-tRNA to the ribosome, e/aIF2 ensures specificity of initiation codon selection. The three subunits of aIF2 from the hyperthermophilic archaeon Pyrococcus abyssi could be overproduced in Escherichia coli. The beta and gamma subunits each contain a tightly bound zinc. The large gamma subunit is shown to form the structural core for trimer assembly. The crystal structures of aIF2gamma, free or complexed to GDP-Mg(2+) or GDPNP-Mg(2+), were resolved at resolutions better than 2 A. aIF2gamma displays marked similarities to elongation factors. A distinctive feature of e/aIF2gamma is a subdomain containing a zinc-binding knuckle. Examination of the nucleotide-complexed aIF2gamma structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor. Implications for the mechanism of translation initiation in both eukarya and archaea are discussed. In particular, positioning of the initiator tRNA in the ribosomal A site during the search for the initiation codon is envisaged. | | The line below this paragraph, {{ABSTRACT_PUBMED_11927566}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11927566 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11927566}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Schmitt, E.]] | | [[Category: Schmitt, E.]] |
| [[Category: Initiation of translation]] | | [[Category: Initiation of translation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:50:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:27:29 2008'' |
