1lb7: Difference between revisions

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[[Image:1lb7.~~jpg~~|left|200px]]

[[Image:1lb7.png|left|200px]]

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~~'''~~IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1~~'''~~

===IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1===

~~==Overview==~~

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~~A panel of 22 naive peptide libraries was constructed in a polyvalent phage display format and sorted against insulin~~-~~like growth factor~~-~~1 (IGF-1).~~ The ~~libraries were pooled to achieve a total diversity of 4.4 x 10(11). After three rounds of selection~~, the ~~majority of~~ the ~~phage clones bound specifically to IGF-1, with a disulfide-constrained CX~~(~~9)C scaffold dominating the selection. Four monovalently displayed sub-libraries were designed~~ on ~~the basis of these conserved motifs~~. Sub-library maturation in a monovalent format yielded an antagonistic peptide that inhibited the interactions between IGF-1 and two cell-surface receptors and those between IGF-1 and two soluble IGF binding proteins with micromolar potency. ~~NMR analysis revealed that the peptide~~ is ~~highly structured in~~ the ~~absence of IGF~~-~~1, and peptides that preorganize the binding elements were selected during the sorting.~~

The line below this paragraph, {{ABSTRACT_PUBMED_11983338}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 11983338 is the PubMed ID number.

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==About this Structure==

Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].

Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].

==Reference==

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[[Category: Loop-helix]]

[[Category: Peptide]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 23:44:46 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 12:12:35 2008''

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-[[Image:1lb7.jpg|left|200px]]
+{{Seed}}
+[[Image:1lb7.png|left|200px]]
 <!--
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 {{STRUCTURE_1lb7|  PDB=1lb7  |  SCENE=  }}
-'''IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1'''
+===IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1===
-==Overview==
+<!--
-A panel of 22 naive peptide libraries was constructed in a polyvalent phage display format and sorted against insulin-like growth factor-1 (IGF-1). The libraries were pooled to achieve a total diversity of 4.4 x 10(11). After three rounds of selection, the majority of the phage clones bound specifically to IGF-1, with a disulfide-constrained CX(9)C scaffold dominating the selection. Four monovalently displayed sub-libraries were designed on the basis of these conserved motifs. Sub-library maturation in a monovalent format yielded an antagonistic peptide that inhibited the interactions between IGF-1 and two cell-surface receptors and those between IGF-1 and two soluble IGF binding proteins with micromolar potency. NMR analysis revealed that the peptide is highly structured in the absence of IGF-1, and peptides that preorganize the binding elements were selected during the sorting.
+The line below this paragraph, {{ABSTRACT_PUBMED_11983338}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 11983338 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_11983338}}
 ==About this Structure==
-Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].
+Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].
 ==Reference==
@@ Line 29: / Line 33: @@
 [[Category: Loop-helix]]
 [[Category: Peptide]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:44:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 12:12:35 2008''