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| '''Crystal structure of tetrameric KaiB from T.elongatus BP-1'''
| | ===Crystal structure of tetrameric KaiB from T.elongatus BP-1=== |
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| ==Overview==
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| KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB consists of 108 amino-acid residues and has a molecular weight of 12 025 Da. KaiB and Cys-substituted KaiB mutants from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 were expressed as GST-fusion proteins in Escherichia coli, purified and crystallized. The crystals of wild-type KaiB belong to the monoclinic space group P2(1), with unit-cell parameters a = 89.6, b = 71.2, c = 106.8 A, beta = 100.1 degrees. While the native crystals diffract to 3.7 A, osmium derivatives, which show an approximately 4 A shrinkage in the b axis, diffract to 2.6 A. The crystals of the singly Cys-substituted mutant T64C with Hg, which show different morphology, diffract to 2.5 A and belong to the monoclinic space group P2, with unit-cell parameters a = 63.7, b = 33.4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination.
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| | {{ABSTRACT_PUBMED_16227211}} |
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| ==About this Structure== | | ==About this Structure== |
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| ==Reference== | | ==Reference== |
| Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1., Iwase R, Imada K, Hayashi F, Uzumaki T, Namba K, Ishiura M, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):727-9. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039567 15039567]
| | Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex., Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, Furukawa Y, Namba K, Ishiura M, J Biol Chem. 2005 Dec 30;280(52):43141-9. Epub 2005 Oct 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16227211 16227211] |
| [[Category: Bacteria]] | | [[Category: Bacteria]] |
| [[Category: Single protein]] | | [[Category: Single protein]] |
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| [[Category: Uzumaki, T.]] | | [[Category: Uzumaki, T.]] |
| [[Category: Circadian clock protein]] | | [[Category: Circadian clock protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:31:07 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 20:35:25 2008'' |
