1ll7: Difference between revisions

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[[Image:1ll7.gif|left|200px]]
{{Seed}}
[[Image:1ll7.png|left|200px]]


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{{STRUCTURE_1ll7|  PDB=1ll7  |  SCENE=  }}  
{{STRUCTURE_1ll7|  PDB=1ll7  |  SCENE=  }}  


'''STRUCTURE OF THE E171Q MUTANT OF C. IMMITIS CHITINASE 1'''
===STRUCTURE OF THE E171Q MUTANT OF C. IMMITIS CHITINASE 1===




==Overview==
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Allosamidin is a known inhibitor of class 18 chitinases. We show that allosamidin is a competitive inhibitor of the fungal chitinase CiX1 from Coccidioides immitis, with a K(i) of 60 nM. We report the X-ray structure of the complex and show that upon inhibitor binding the side-chain of Asp169 rotates to form an ion pair with the oxazolinium cation. The mechanism of action is thought to involve protonation of the leaving group by Glu171 and substrate assistance by the sugar acetamido moiety to form an oxazoline-like intermediate. We converted both amino acid residues to the corresponding amide and found that each mutation effectively abolishes enzyme activity. X-ray structures show the mutant enzymes retain the basic wild-type structure and that the loss of mutant activity is due to their altered chemical properties. The high affinity of allosamidin, and its similarity to the putative reaction intermediate, suggests it is a transition state analog. This helps validate our contention that the role of Asp169 is to electrostatically stabilize the reaction transition state.
The line below this paragraph, {{ABSTRACT_PUBMED_12079386}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12079386}}


==About this Structure==
==About this Structure==
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[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
[[Category: Beta-alpha barrel]]
[[Category: Beta-alpha barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:01:50 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:21:54 2008''

Revision as of 18:21, 2 July 2008

File:1ll7.png

Template:STRUCTURE 1ll7

STRUCTURE OF THE E171Q MUTANT OF C. IMMITIS CHITINASE 1

Template:ABSTRACT PUBMED 12079386

About this Structure

1LL7 is a Single protein structure of sequence from Coccidioides immitis. Full crystallographic information is available from OCA.

Reference

The structure of an allosamidin complex with the Coccidioides immitis chitinase defines a role for a second acid residue in substrate-assisted mechanism., Bortone K, Monzingo AF, Ernst S, Robertus JD, J Mol Biol. 2002 Jul 5;320(2):293-302. PMID:12079386

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