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| [[Image:1m31.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1m31| PDB=1m31 | SCENE= }} | | {{STRUCTURE_1m31| PDB=1m31 | SCENE= }} |
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| '''Three-Dimensional Solution Structure of Apo-Mts1'''
| | ===Three-Dimensional Solution Structure of Apo-Mts1=== |
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| ==Overview==
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| Mts1 is a member of the S100 family of Ca2+-binding proteins and is implicated in promoting tumor progression and metastasis. To better understand the structure-function relationships of this protein and to begin characterizing its Ca2+-dependent interaction with protein binding targets, the three-dimensional structure of mts1 was determined in the apo state by NMR spectroscopy. As with other S100 protein family members, mts1 is a symmetric homodimer held together by noncovalent interactions between two helices from each subunit (helices 1, 4, 1', and 4') to form an X-type four-helix bundle. Each subunit of mts1 has two EF-hand Ca2+-binding domains: a pseudo-EF-hand (or S100-hand) and a typical EF-hand that are brought into proximity by a small two-stranded antiparallel beta-sheet. The S100-hand is formed by helices 1 and 2, and is similar in conformation to other members of the S100 family. In the typical EF-hand, the position of helix 3 is similar to that of another member of the S100 protein family, calcyclin (S100A6), and less like that of other S100 family members for which three-dimensional structures are available in the calcium-free state (e.g., S100B and S100A1). The differences in the position of helix 3 in the apo state of these four S100 proteins are likely due to variations in the amino acid sequence in the C-terminus of helix 4 and in loop 2 (the hinge region) and could potentially be used to subclassify the S100 protein family.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12379109}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12379109 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12379109}} |
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| ==About this Structure== | | ==About this Structure== |
| 1M31 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M31 OCA]. | | 1M31 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M31 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Non-covalent homodimer]] | | [[Category: Non-covalent homodimer]] |
| [[Category: X-type four-helix bundle]] | | [[Category: X-type four-helix bundle]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:34:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:06:40 2008'' |
