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| {{STRUCTURE_1m4w| PDB=1m4w | SCENE= }} | | {{STRUCTURE_1m4w| PDB=1m4w | SCENE= }} |
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| '''Thermophilic b-1,4-xylanase from Nonomuraea flexuosa'''
| | ===Thermophilic b-1,4-xylanase from Nonomuraea flexuosa=== |
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| ==Overview==
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| The crystal structures of thermophilic xylanases from Chaetomium thermophilum and Nonomuraea flexuosa were determined at 1.75 and 2.1 A resolution, respectively. Both enzymes have the overall fold typical to family 11 xylanases with two highly twisted beta-sheets forming a large cleft. The comparison of 12 crystal structures of family 11 xylanases from both mesophilic and thermophilic organisms showed that the structures of different xylanases are very similar. The sequence identity differences correlated well with the structural differences. Several minor modifications appeared to be responsible for the increased thermal stability of family 11 xylanases: (a) higher Thr : Ser ratio (b) increased number of charged residues, especially Arg, resulting in enhanced polar interactions, and (c) improved stabilization of secondary structures involved the higher number of residues in the beta-strands and stabilization of the alpha-helix region. Some members of family 11 xylanases have a unique strategy to improve their stability, such as a higher number of ion pairs or aromatic residues on protein surface, a more compact structure, a tighter packing, and insertions at some regions resulting in enhanced interactions. | | The line below this paragraph, {{ABSTRACT_PUBMED_12653995}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12653995 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12653995}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Thermostability]] | | [[Category: Thermostability]] |
| [[Category: Xylanase]] | | [[Category: Xylanase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:38:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:12:54 2008'' |
