1m5n: Difference between revisions

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[[Image:1m5n.~~gif~~|left|200px]]

[[Image:1m5n.png|left|200px]]

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~~'''~~Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP~~'''~~

===Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP===

~~==Overview==~~

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~~Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha~~, ~~which associates with importin beta via the IBB domain. Other proteins~~, ~~including parathyroid hormone-related protein (PTHrP), are imported into~~ the ~~nucleus by direct interaction with importin beta. We solved~~ the ~~crystal structure of a fragment of importin beta-1~~ (~~1-485) bound to the nonclassical NLS of PTHrP~~. ~~The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site~~. ~~Using a permeabilized cell import assay we demonstrate that importin beta (1-485~~) ~~can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain~~, ~~which could have evolved into~~ the ~~modern importin beta superfamily~~.

The line below this paragraph, {{ABSTRACT_PUBMED_12504010}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12504010 is the PubMed ID number.

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==About this Structure==

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[[Category: All helical protein]]

[[Category: Heat repeat]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ ~~3 00~~:39:48 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:15:52 2008''

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-[[Image:1m5n.gif|left|200px]]
+{{Seed}}
+[[Image:1m5n.png|left|200px]]
 <!--
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 {{STRUCTURE_1m5n|  PDB=1m5n  |  SCENE=  }}
-'''Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP'''
+===Crystal structure of HEAT repeats (1-11) of importin b bound to the non-classical NLS(67-94) of PTHrP===
-==Overview==
+<!--
-Nuclear import of proteins containing a classical nuclear localization signal (NLS) involves NLS recognition by importin alpha, which associates with importin beta via the IBB domain. Other proteins, including parathyroid hormone-related protein (PTHrP), are imported into the nucleus by direct interaction with importin beta. We solved the crystal structure of a fragment of importin beta-1 (1-485) bound to the nonclassical NLS of PTHrP. The structure reveals a second extended cargo binding site on importin beta distinct from the IBB domain binding site. Using a permeabilized cell import assay we demonstrate that importin beta (1-485) can import PTHrP-coupled cargo in a Ran-dependent manner. We propose that this region contains a prototypical nuclear import receptor domain, which could have evolved into the modern importin beta superfamily.
+The line below this paragraph, {{ABSTRACT_PUBMED_12504010}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12504010 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12504010}}
 ==About this Structure==
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 [[Category: All helical protein]]
 [[Category: Heat repeat]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:39:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 23:15:52 2008''