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| [[Image:1m6s.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1m6s| PDB=1m6s | SCENE= }} | | {{STRUCTURE_1m6s| PDB=1m6s | SCENE= }} |
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| '''Crystal Structure Of Threonine Aldolase'''
| | ===Crystal Structure Of Threonine Aldolase=== |
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| ==Overview==
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| L-Threonine acetaldehyde-lyase (threonine aldolase, TA) is a pyridoxal-5'-phosphate-dependent (PLP) enzyme that catalyzes conversion of L-threonine or L-allo-threonine to glycine and acetaldehyde in a secondary glycine biosynthetic pathway. X-ray structures of Thermatoga maritima TA have been determined as the apo-enzyme at 1.8 A resolution and bound to substrate L-allo-threonine and product glycine at 1.9 and 2.0 A resolution, respectively. Despite low pairwise sequence identities, TA is a member of aspartate aminotransferase (AATase) fold family of PLP enzymes. The enzyme forms a 222 homotetramer with the PLP cofactor bound via a Schiff-base linkage to Lys199 within a domain interface. The structure reveals bound calcium and chloride ions that appear to contribute to catalysis and oligomerization, respectively. Although L-threonine and L-allo-threonine are substrates for T. maritima TA, enzymatic assays revealed a strong preference for L-allo-threonine. Structures of the external aldimines with substrate/product reveal a pair of histidines that may provide flexibility in substrate recognition. Variation in the threonine binding pocket may explain preferences for L-allo-threonine versus L-threonine among TA family members.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12269813}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12269813 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12269813}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Threonine]] | | [[Category: Threonine]] |
| [[Category: Vitamin b12]] | | [[Category: Vitamin b12]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:42:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:20:10 2008'' |
