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| [[Image:1mb9.gif|left|200px]] | | {{Seed}} |
| | [[Image:1mb9.png|left|200px]] |
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| {{STRUCTURE_1mb9| PDB=1mb9 | SCENE= }} | | {{STRUCTURE_1mb9| PDB=1mb9 | SCENE= }} |
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| '''BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP'''
| | ===BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP=== |
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| ==Overview==
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| The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12409610}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12409610 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12409610}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Clavulanic acid]] | | [[Category: Clavulanic acid]] |
| [[Category: Deoxyguanidinoproclavaminic acid]] | | [[Category: Deoxyguanidinoproclavaminic acid]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:51:02 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:36:42 2008'' |
