1mfa: Difference between revisions

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[[Image:1mfa.~~gif~~|left|200px]]

[[Image:1mfa.png|left|200px]]

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~~'''~~STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION~~'''~~

===STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION===

~~==Overview==~~

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~~We describe here the 1.7~~-~~A resolution structure of a single~~-~~chain antibody variable domain (scFv) molecule~~, ~~based on~~ the ~~carbohydrate-binding antibody Se155-4, complexed with~~ the ~~trisaccharide ligand alpha-D-Gal~~(~~1-->2~~)~~[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe~~, where ~~Abe~~ is ~~abequose. The scFv expressed in Escherichia coli has~~ the ~~variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker~~. ~~Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man~~-~~Gal linkage than was observed previously in the Fab~~-~~trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.~~

The line below this paragraph, {{ABSTRACT_PUBMED_7517550}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 7517550 is the PubMed ID number.

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==About this Structure==

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[[Category: Zdanov, A.]]

[[Category: Immunoglobulin]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May~~ ~~3 00~~:57:52 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:50:34 2008''

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-[[Image:1mfa.gif|left|200px]]
+{{Seed}}
+[[Image:1mfa.png|left|200px]]
 <!--
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 {{STRUCTURE_1mfa|  PDB=1mfa  |  SCENE=  }}
-'''STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION'''
+===STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION===
-==Overview==
+<!--
-We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1--&gt;2)[alpha-D-Abe(1--&gt;3)]alpha-D-Manp1--&gt;OMe, where Abe is abequose. The scFv expressed in Escherichia coli has the variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker. Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man-Gal linkage than was observed previously in the Fab-trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.
+The line below this paragraph, {{ABSTRACT_PUBMED_7517550}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 7517550 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_7517550}}
 ==About this Structure==
@@ Line 23: / Line 27: @@
 [[Category: Zdanov, A.]]
 [[Category: Immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:57:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  2 23:50:34 2008''