|
|
| Line 1: |
Line 1: |
| [[Image:1mmf.gif|left|200px]] | | {{Seed}} |
| | [[Image:1mmf.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1mmf| PDB=1mmf | SCENE= }} | | {{STRUCTURE_1mmf| PDB=1mmf | SCENE= }} |
|
| |
|
| '''Crystal structure of substrate free form of glycerol dehydratase'''
| | ===Crystal structure of substrate free form of glycerol dehydratase=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Glycerol dehydratase (GDH) and diol dehydratase (DDH) are highly homologous isofunctional enzymes that catalyze the elimination of water from glycerol and 1,2-propanediol (1,2-PD) to the corresponding aldehyde via a coenzyme B(12)-dependent radical mechanism. The crystal structure of substrate free form of GDH in complex with cobalamin and K(+) has been determined at 2.5 A resolution. Its overall fold and the subunit assembly closely resemble those of DDH. Comparison of this structure and the DDH structure, available only in substrate bound form, shows the expected change of the coordination of the essential K(+) from hexacoordinate to heptacoordinate with the displacement of a single coordinated water by the substrate diol. In addition, there appears to be an increase in the rigidity of the K(+) coordination (as measured by lower B values) upon the binding of the substrate. Structural analysis of the locations of conserved residues among various GDH and DDH sequences has aided in identification of residues potentially important for substrate preference or specificity of protein-protein interactions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12538056}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12538056 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_12538056}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 32: |
Line 36: |
| [[Category: Glycerol dehydratase]] | | [[Category: Glycerol dehydratase]] |
| [[Category: Tim barrel]] | | [[Category: Tim barrel]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:25:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:21:16 2008'' |
