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| [[Image:1mq1.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1mq1| PDB=1mq1 | SCENE= }} | | {{STRUCTURE_1mq1| PDB=1mq1 | SCENE= }} |
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| '''Ca2+-S100B-TRTK-12 complex'''
| | ===Ca2+-S100B-TRTK-12 complex=== |
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| ==Overview==
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| The Alzheimer-linked neural protein S100B is a signaling molecule shown to control the assembly of intermediate filament proteins in a calcium-sensitive manner. Upon binding calcium, a conformational change occurs in S100B exposing a hydrophobic surface for target protein interactions. The synthetic peptide TRTK-12 (TRTKIDWNKILS), derived from random bacteriophage library screening, bears sequence similarity to several intermediate filament proteins and has the highest calcium-dependent affinity of any target molecule for S100B to date (K(d) <1 microm). In this work, the three-dimensional structure of the Ca(2+)-S100B-TRTK-12 complex has been determined by NMR spectroscopy. The structure reveals an extended, contiguous hydrophobic surface is formed on Ca(2+)-S100B for target interaction. The TRTK-12 peptide adopts a coiled structure that fits into a portion of this surface, anchored at Trp(7), and interacts with multiple hydrophobic contacts in helices III and IV of Ca(2+)-S100B. This interaction is strikingly different from the alpha-helical structures found for other S100 target peptides. By using the TRTK-12 interaction as a guide, in combination with other available S100 target structures, a recognition site on helix I is identified that may act in concert with the TRTK-12-binding site from helices III and IV. This would provide a larger, more complex site to interact with full-length target proteins and would account for the promiscuity observed for S100B target protein interactions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12480931}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12480931 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12480931}} |
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| ==About this Structure== | | ==About this Structure== |
| 1MQ1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ1 OCA]. | | 1MQ1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ1 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Ef-hand]] | | [[Category: Ef-hand]] |
| [[Category: Protein-peptide complex]] | | [[Category: Protein-peptide complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:34:47 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:48:50 2008'' |
