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| {{STRUCTURE_3ran| PDB=3ran | SCENE= }} | | {{STRUCTURE_3ran| PDB=3ran | SCENE= }} |
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| '''CANINE GDP-RAN Q69L MUTANT'''
| | ===CANINE GDP-RAN Q69L MUTANT=== |
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| ==Overview==
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| We report the 2.3 A resolution X-ray crystal structure of the GDP-bound form of the RanQ69L mutant that is used extensively in studies of nucleocytoplasmic transport and cell-cycle progression. When the structure of GDP-RanQ69L from monoclinic crystals with P21 symmetry was compared with the structure of wild-type Ran obtained from monoclinic crystals, the Q69L mutant showed a large conformational change in residues 68-74, which are in the switch II region of the molecule which changes conformation in response to nucleotide state and which forms the major interaction interface with nuclear transport factor 2 (NTF2, sometimes called p10). This conformational change alters the positions of key residues such as Lys71, Phe72 and Arg76 that are crucial for the interaction of GDP-Ran with NTF2 and indeed, solution binding studies were unable to detect any interaction between NTF2 and GDP-RanQ69L under conditions where GDP-Ran bound effectively. This interaction between NTF2 and GDP-Ran is required for efficient nuclear protein import and may function between the docking and translocation steps of the pathway.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9878368}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9878368 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9878368}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Gtpase]] | | [[Category: Gtpase]] |
| [[Category: Nuclear transport]] | | [[Category: Nuclear transport]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:11:47 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:04:45 2008'' |
