4gch: Difference between revisions

Line 1:

[[Image:4gch.~~jpg~~|left|200px]]

[[Image:4gch.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~STRUCTURE AND ACTIVITY OF TWO PHOTOREVERSIBLE CINNAMATES BOUND TO CHYMOTRYPSIN~~'''~~

===STRUCTURE AND ACTIVITY OF TWO PHOTOREVERSIBLE CINNAMATES BOUND TO CHYMOTRYPSIN===

~~==Overview==~~

<!--

The ~~serine protease gamma-chymotrypsin was covalently inhibited with two different photoreversible cinnamate compounds~~, ~~and~~ the ~~structures of~~ the ~~resulting complexes were determined to 1~~.~~9-A resolution~~. ~~The inhibitors show different kinetics of binding~~, ~~inhibition, and nonphotochemical deacylation relative to each other in solution activity assays~~. ~~The crystal structures of the enzyme~~-cinnamate complexes show that both compounds acylate serine 195 and that the two molecules are bound in similar nonproductive conformations which have drastic effects on their ability to turn over. Substitution of a diethylamino group on the para position of the cinnamate ring causes a 1000-~~fold increase in the thermal stability of the inhibitor toward hydrolysis and deacylation.~~

The line below this paragraph, {{ABSTRACT_PUBMED_2364065}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 2364065 is the PubMed ID number.

-->

==About this Structure==

Line 26:

Line 30:

[[Category: Ringe, D.]]

[[Category: Stoddard, B L.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:24:25 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:38:22 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:4gch.jpg|left|200px]]
+{{Seed}}
+[[Image:4gch.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_4gch|  PDB=4gch  |  SCENE=  }}
-'''STRUCTURE AND ACTIVITY OF TWO PHOTOREVERSIBLE CINNAMATES BOUND TO CHYMOTRYPSIN'''
+===STRUCTURE AND ACTIVITY OF TWO PHOTOREVERSIBLE CINNAMATES BOUND TO CHYMOTRYPSIN===
-==Overview==
+<!--
-The serine protease gamma-chymotrypsin was covalently inhibited with two different photoreversible cinnamate compounds, and the structures of the resulting complexes were determined to 1.9-A resolution. The inhibitors show different kinetics of binding, inhibition, and nonphotochemical deacylation relative to each other in solution activity assays. The crystal structures of the enzyme-cinnamate complexes show that both compounds acylate serine 195 and that the two molecules are bound in similar nonproductive conformations which have drastic effects on their ability to turn over. Substitution of a diethylamino group on the para position of the cinnamate ring causes a 1000-fold increase in the thermal stability of the inhibitor toward hydrolysis and deacylation.
+The line below this paragraph, {{ABSTRACT_PUBMED_2364065}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 2364065 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_2364065}}
 ==About this Structure==
@@ Line 26: / Line 30: @@
 [[Category: Ringe, D.]]
 [[Category: Stoddard, B L.]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:24:25 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul  3 13:38:22 2008''