4lyo: Difference between revisions

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[[Image:4lyo.jpg|left|200px]]
{{Seed}}
[[Image:4lyo.png|left|200px]]


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{{STRUCTURE_4lyo|  PDB=4lyo  |  SCENE=  }}  
{{STRUCTURE_4lyo|  PDB=4lyo  |  SCENE=  }}  


'''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER'''
===CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER===




==Overview==
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Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.
The line below this paragraph, {{ABSTRACT_PUBMED_9659395}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9659395 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9659395}}


==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:26:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:43:21 2008''

Revision as of 10:43, 3 July 2008

File:4lyo.png

Template:STRUCTURE 4lyo

CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER

Template:ABSTRACT PUBMED 9659395

About this Structure

4LYO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:9659395

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