6msf: Difference between revisions

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[[Image:6msf.gif|left|200px]]
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[[Image:6msf.png|left|200px]]


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{{STRUCTURE_6msf|  PDB=6msf  |  SCENE=  }}  
{{STRUCTURE_6msf|  PDB=6msf  |  SCENE=  }}  


'''F6 APTAMER MS2 COAT PROTEIN COMPLEX'''
===F6 APTAMER MS2 COAT PROTEIN COMPLEX===




==Overview==
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The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures.
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{{ABSTRACT_PUBMED_9461079}}


==About this Structure==
==About this Structure==
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[[Category: Rna stem loop]]
[[Category: Rna stem loop]]
[[Category: Viral coat protein/rna complex]]
[[Category: Viral coat protein/rna complex]]
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Revision as of 11:22, 3 July 2008

File:6msf.png

Template:STRUCTURE 6msf

F6 APTAMER MS2 COAT PROTEIN COMPLEX

Template:ABSTRACT PUBMED 9461079

About this Structure

6MSF is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

Reference

Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution., Convery MA, Rowsell S, Stonehouse NJ, Ellington AD, Hirao I, Murray JB, Peabody DS, Phillips SE, Stockley PG, Nat Struct Biol. 1998 Feb;5(2):133-9. PMID:9461079

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