2knt: Difference between revisions

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[[Image:2knt.~~gif~~|left|200px]]

[[Image:2knt.png|left|200px]]

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~~'''~~THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5~~'''~~

===THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5===

~~==Overview==~~

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The ~~recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain was previously described at 1.6 A resolution at room temperature. By changing~~ the ~~crystallization conditions and using synchrotron radiation, we are able to record diffraction data~~ to ~~1.2 A resolution for crystals of~~ the ~~same space group~~ at ~~291 K~~. ~~The protein-water-ion model has been refined anisotropically against these new data using the program SHELXL93; the results converged to an R factor of 15~~.0%, ~~with all data between 7 and 1~~.~~2 A. The final electron~~-~~density map reveals a clear chain tracing with a few disordered residues and five residues out of 58 that present alternate conformations. The Cys14~~-Cys38 bond presents the less frequently observed left-hand conformation (chi1 = -60 degrees). The solvent molecules and a phosphate ion are well ordered with an average B of 38 A2. The high-resolution structure reveals the N and C termini which were missing from the 1.6 A structure.

The line below this paragraph, {{ABSTRACT_PUBMED_9761897}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 9761897 is the PubMed ID number.

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==About this Structure==

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[[Category: Extracellular matrix]]

[[Category: Kunitz inhibitor]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 09~~:29:21 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat Jul 5 16:24:44 2008''

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-[[Image:2knt.gif|left|200px]]
+{{Seed}}
+[[Image:2knt.png|left|200px]]
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 {{STRUCTURE_2knt|  PDB=2knt  |  SCENE=  }}
-'''THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5'''
+===THE 1.2 ANGSTROM STRUCTURE OF KUNITZ TYPE DOMAIN C5===
-==Overview==
+<!--
-The recombinant Kunitz-type domain (C5) of human collagen alpha3(VI) chain was previously described at 1.6 A resolution at room temperature. By changing the crystallization conditions and using synchrotron radiation, we are able to record diffraction data to 1.2 A resolution for crystals of the same space group at 291 K. The protein-water-ion model has been refined anisotropically against these new data using the program SHELXL93; the results converged to an R factor of 15.0%, with all data between 7 and 1.2 A. The final electron-density map reveals a clear chain tracing with a few disordered residues and five residues out of 58 that present alternate conformations. The Cys14-Cys38 bond presents the less frequently observed left-hand conformation (chi1 = -60 degrees). The solvent molecules and a phosphate ion are well ordered with an average B of 38 A2. The high-resolution structure reveals the N and C termini which were missing from the 1.6 A structure.
+The line below this paragraph, {{ABSTRACT_PUBMED_9761897}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 9761897 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_9761897}}
 ==About this Structure==
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 [[Category: Extracellular matrix]]
 [[Category: Kunitz inhibitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:29:21 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat Jul  5 16:24:44 2008''