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| [[Image:2avi.gif|left|200px]] | | {{Seed}} |
| | [[Image:2avi.png|left|200px]] |
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| {{STRUCTURE_2avi| PDB=2avi | SCENE= }} | | {{STRUCTURE_2avi| PDB=2avi | SCENE= }} |
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| '''THREE-DIMENSIONAL STRUCTURES OF AVIDIN AND THE AVIDIN-BIOTIN COMPLEX'''
| | ===THREE-DIMENSIONAL STRUCTURES OF AVIDIN AND THE AVIDIN-BIOTIN COMPLEX=== |
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| ==Overview==
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| The crystal structures of a deglycosylated form of the egg-white glycoprotein avidin and of its complex with biotin have been determined to 2.6 and 3.0 A, respectively. The structures reveal the amino acid residues critical for stabilization of the tetrameric assembly and for the exceptionally tight binding of biotin. Each monomer is an eight-stranded antiparallel beta-barrel, remarkably similar to that of the genetically distinct bacterial analog streptavidin. As in streptavidin, binding of biotin involves a highly stabilized network of polar and hydrophobic interactions. There are, however, some differences. The presence of additional hydrophobic and hydrophilic groups in the binding site of avidin (which are missing in streptavidin) may account for its higher affinity constant. Two amino acid substitutions are proposed to be responsible for its susceptibility to denaturation relative to streptavidin. Unexpectedly, a residual N-acetylglucosamine moiety was detected in the deglycosylated avidin monomer by difference Fourier synthesis. | | The line below this paragraph, {{ABSTRACT_PUBMED_8506353}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8506353 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8506353}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sussman, J.]] | | [[Category: Sussman, J.]] |
| [[Category: Biotin binding protein]] | | [[Category: Biotin binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:31:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 6 12:05:31 2008'' |
