2cld: Difference between revisions

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[[Image:2cld.gif|left|200px]]
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[[Image:2cld.png|left|200px]]


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{{STRUCTURE_2cld|  PDB=2cld  |  SCENE=  }}  
{{STRUCTURE_2cld|  PDB=2cld  |  SCENE=  }}  


'''CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP (2)'''
===CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP (2)===




==Overview==
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We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normally without the need of a specialized mechanical support. As a biological model system, we have used H-Ras p21 with an artificially introduced photo-labile GTP precursor (caged GTP) and a covalently attached fluorophore (IANBD amide). Using the FLUMIX system, detailed information about the state of photolyzed crystals of the modified H-Ras p21 (p21(mod)) could be obtained. Measurements in combination with a synchrotron beamline showed significant fluorescence changes in p21(mod) crystals even within a few seconds of x-ray exposure at 100 K.
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{{ABSTRACT_PUBMED_16698776}}


==About this Structure==
==About this Structure==
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[[Category: Proto-oncogene]]
[[Category: Proto-oncogene]]
[[Category: R-caged gtp]]
[[Category: R-caged gtp]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:25:08 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 12:39:27 2008''

Revision as of 09:39, 10 July 2008

File:2cld.png

Template:STRUCTURE 2cld

CRYSTAL STRUCTURE ANALYSIS OF A FLUORESCENT FORM OF H-RAS P21 IN COMPLEX WITH GDP (2)

Template:ABSTRACT PUBMED 16698776

About this Structure

2CLD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction., Klink BU, Goody RS, Scheidig AJ, Biophys J. 2006 Aug 1;91(3):981-92. Epub 2006 May 12. PMID:16698776

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