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| [[Image:2v0r.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2v0r| PDB=2v0r | SCENE= }} | | {{STRUCTURE_2v0r| PDB=2v0r | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LTX) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE'''
| | ===CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LTX) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE=== |
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| ==Overview==
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| The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of new L1 and Alu elements that presently account for approximately 28% of the human genome. L1-EN bears considerable technological interest, because its target selectivity may ultimately be engineered to allow the site-specific integration of DNA into defined genomic locations. Based on the crystal structure, we generated L1-EN mutants to analyze and manipulate DNA target site recognition. Crystal structures and their dynamic and functional analysis show entire loop grafts to be feasible, resulting in altered specificity, while individual point mutations do not change the nicking pattern of L1-EN. Structural parameters of the DNA target seem more important for recognition than the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro are less well defined than the respective integration site consensus in vivo. This suggests that additional factors other than the DNA nicking specificity of L1-EN contribute to the targeted integration of non-LTR retrotransposons. | | The line below this paragraph, {{ABSTRACT_PUBMED_17626046}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17626046 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17626046}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Retrotransposon]] | | [[Category: Retrotransposon]] |
| [[Category: Transcription]] | | [[Category: Transcription]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:59:21 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 12:51:55 2008'' |
