1oo4: Difference between revisions

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[[Image:1oo4.gif|left|200px]]
{{Seed}}
[[Image:1oo4.png|left|200px]]


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{{STRUCTURE_1oo4|  PDB=1oo4  |  SCENE=  }}  
{{STRUCTURE_1oo4|  PDB=1oo4  |  SCENE=  }}  


'''P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr'''
===P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase complexed to a peptide derived from PDGFr===




==Overview==
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Understanding the specificity of Src homology 2 (SH2) domains is important because of their critical role in cell signaling. Previous genetic analysis has characterized mutants of the N-terminal src homology 2 (SH2) domain of the p85 subunit of phosphoinositide 3-kinase (PI3K). The P395S mutant exhibits a specificity for phosphopeptide binding different from that of the wild-type SH2. The P395S mutant has an increased affinity for the platelet-derived growth factor receptor (PDGFr) compared to polyomavirus middle T antigen (MT). Solution structures of the P395S mutant of the p85 N-SH2 alone and complexed to a PDGFr phosphopeptide were determined to explain the change in specificity. Chemical shift perturbations caused by different peptides were compared for mutant and wild-type structures. The results show that the single P395S mutation has broad effects on the structure. Furthermore, they provide a rationale for the observed changes in binding preference.
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{{ABSTRACT_PUBMED_14503862}}


==About this Structure==
==About this Structure==
1OO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO4 OCA].  
1OO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO4 OCA].  


==Reference==
==Reference==
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[[Category: Pdgfr complex]]
[[Category: Pdgfr complex]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
[[Category: Src homology 2 domain p85 regulatory subunit mutant]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:05:28 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:26:58 2008''
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