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| {{STRUCTURE_2doq| PDB=2doq | SCENE= }} | | {{STRUCTURE_2doq| PDB=2doq | SCENE= }} |
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| '''crystal structure of Sfi1p/Cdc31p complex'''
| | ===crystal structure of Sfi1p/Cdc31p complex=== |
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| ==Overview==
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| Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16785321}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16785321 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16785321}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sfi1p]] | | [[Category: Sfi1p]] |
| [[Category: Spindle pole body]] | | [[Category: Spindle pole body]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:53:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:36:00 2008'' |
