2rhs: Difference between revisions

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[[Image:2rhs.~~jpg~~|left|200px]]

[[Image:2rhs.png|left|200px]]

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~~'''~~PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies~~'''~~

===PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies===

~~==Overview==~~

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In this ~~article~~, ~~we describe for~~ the ~~first time~~ the ~~high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus~~. ~~We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog~~. ~~We also explain~~ the ~~structure-activity relationship of several recently reported inhibitors~~. ~~The native enzyme crystals were of poor quality~~--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.

The line below this paragraph, {{ABSTRACT_PUBMED_18086534}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18086534 is the PubMed ID number.

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==About this Structure==

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[[Category: Trna]]

[[Category: Trna-binding]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed May 7 08~~:51:26 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:41:50 2008''

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-[[Image:2rhs.jpg|left|200px]]
+{{Seed}}
+[[Image:2rhs.png|left|200px]]
 <!--
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 {{STRUCTURE_2rhs|  PDB=2rhs  |  SCENE=  }}
-'''PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies'''
+===PheRS from Staphylococcus haemolyticus- rational protein engineering and inhibitor studies===
-==Overview==
+<!--
-In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog. We also explain the structure-activity relationship of several recently reported inhibitors. The native enzyme crystals were of poor quality--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.
+The line below this paragraph, {{ABSTRACT_PUBMED_18086534}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18086534 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18086534}}
 ==About this Structure==
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 [[Category: Trna]]
 [[Category: Trna-binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May  7 08:51:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:41:50 2008''