2hvs: Difference between revisions

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[[Image:2hvs.~~gif~~|left|200px]]

[[Image:2hvs.png|left|200px]]

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~~'''~~Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 2'-deoxyribonucleotide at the nick~~'''~~

===Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 2'-deoxyribonucleotide at the nick===

~~==Overview==~~

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~~T4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a family of RNA repair enzymes that seal 3'OH/5'PO(4) nicks in duplex RNAs via ligase adenylylation (step 1)~~, ~~AMP transfer~~ to the ~~nick 5'PO~~(~~4) (step 2), and attack by the nick 3'OH~~ on ~~the 5'-adenylylated strand to form a phosphodiester (step 3). Crystal structures are reported for Rnl2~~ at ~~discrete steps along this pathway~~: the ~~covalent Rnl2-AMP intermediate; Rnl2 bound to an adenylylated nicked duplex, captured immediately following step 2; and Rnl2 at an adenylylated nick in a state poised for step 3~~. ~~These structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active~~-~~site contacts and conformational changes propel the ligation reaction forward. Mutational analysis and comparison of nick~~-~~bound structures of Rnl2 and human DNA ligase I highlight common and divergent themes of substrate recognition that can explain their specialization for RNA versus DNA repair.~~

The line below this paragraph, {{ABSTRACT_PUBMED_17018278}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 17018278 is the PubMed ID number.

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==About this Structure==

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[[Category: Rna]]

[[Category: T4]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 06~~:46:26 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:43:56 2008''

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-[[Image:2hvs.gif|left|200px]]
+{{Seed}}
+[[Image:2hvs.png|left|200px]]
 <!--
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 {{STRUCTURE_2hvs|  PDB=2hvs  |  SCENE=  }}
-'''Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 2'-deoxyribonucleotide at the nick'''
+===Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 2'-deoxyribonucleotide at the nick===
-==Overview==
+<!--
-T4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a family of RNA repair enzymes that seal 3'OH/5'PO(4) nicks in duplex RNAs via ligase adenylylation (step 1), AMP transfer to the nick 5'PO(4) (step 2), and attack by the nick 3'OH on the 5'-adenylylated strand to form a phosphodiester (step 3). Crystal structures are reported for Rnl2 at discrete steps along this pathway: the covalent Rnl2-AMP intermediate; Rnl2 bound to an adenylylated nicked duplex, captured immediately following step 2; and Rnl2 at an adenylylated nick in a state poised for step 3. These structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active-site contacts and conformational changes propel the ligation reaction forward. Mutational analysis and comparison of nick-bound structures of Rnl2 and human DNA ligase I highlight common and divergent themes of substrate recognition that can explain their specialization for RNA versus DNA repair.
+The line below this paragraph, {{ABSTRACT_PUBMED_17018278}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 17018278 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_17018278}}
 ==About this Structure==
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 [[Category: Rna]]
 [[Category: T4]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 06:46:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:43:56 2008''