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| [[Image:1n9l.png|left|200px]] | | [[Image:1n9l.png|left|200px]] |
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| {{STRUCTURE_1n9l| PDB=1n9l | SCENE= }} | | {{STRUCTURE_1n9l| PDB=1n9l | SCENE= }} |
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| '''Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in the dark state.'''
| | ===Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in the dark state.=== |
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| ==Overview==
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| Phot proteins (phototropins and homologs) are blue-light photoreceptors that control mechanical processes like phototropism, chloroplast relocation, or guard-cell opening in plants. Phot receptors consist of two flavin mononucleotide (FMN)-binding light, oxygen, or voltage (LOV) domains and a C-terminal serine/threonine kinase domain. We determined crystal structures of the LOV1 domain of Phot1 from the green alga Chlamydomonas reinhardtii in the dark and illuminated state to 1.9 A and 2.8 A resolution, respectively. The structure resembles that of LOV2 from Adiantum (Crosson, S. and K. Moffat. 2001. PROC: Natl. Acad. Sci. USA. 98:2995-3000). In the resting dark state of LOV1, the reactive Cys-57 is present in two conformations. Blue-light absorption causes formation of a proposed active signaling state that is characterized by a covalent bond between the flavin C4a and the thiol of Cys-57. There are differences around the FMN chromophore but no large overall conformational changes. Quantum chemical calculations based on the crystal structures revealed the electronic distribution in the active site during the photocycle. The results suggest trajectories for electrons, protons, and the active site cysteine and offer an interpretation of the reaction mechanism.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12668455}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12668455 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12668455}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Phototropin]] | | [[Category: Phototropin]] |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 04:52:17 2008'' | | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:52:06 2008'' |
