2olg: Difference between revisions

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[[Image:2olg.gif|left|200px]]
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[[Image:2olg.png|left|200px]]


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{{STRUCTURE_2olg|  PDB=2olg  |  SCENE=  }}  
{{STRUCTURE_2olg|  PDB=2olg  |  SCENE=  }}  


'''Crystal structure of the serine protease domain of prophenoloxidase activating factor-I in a zymogen form'''
===Crystal structure of the serine protease domain of prophenoloxidase activating factor-I in a zymogen form===




==Overview==
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A family of serine proteases (SPs) mediates the proteolytic cascades of embryonic development and immune response in invertebrates. These proteases, called easter-type SPs, consist of clip and chymotrypsin-like SP domains. The SP domain of easter-type proteases differs from those of typical SPs in its primary structure. Herein, we report the first crystal structure of the SP domain of easter-type proteases, presented as that of prophenoloxidase activating factor (PPAF)-I in zymogen form. This structure reveals several important structural features including a bound calcium ion, an additional loop with a unique disulfide linkage, a canyon-like deep active site, and an exposed activation loop. We subsequently show the role of the bound calcium and the proteolytic susceptibility of the activation loop, which occurs in a clip domain-independent manner. Based on biochemical study in the presence of heparin, we suggest that PPAF-III, highly homologous to PPAF-I, contains a surface patch that is responsible for enhancing the catalytic activity through interaction with a nonsubstrate region of a target protein. These results provide insights into an activation mechanism of easter-type proteases in proteolytic cascades, in comparison with the well studied blood coagulation enzymes in mammals.
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{{ABSTRACT_PUBMED_17287215}}


==About this Structure==
==About this Structure==
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[[Category: Prophenoloxidase activating factor-i]]
[[Category: Prophenoloxidase activating factor-i]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:56:32 2008''

Revision as of 09:56, 27 July 2008

File:2olg.png

Template:STRUCTURE 2olg

Crystal structure of the serine protease domain of prophenoloxidase activating factor-I in a zymogen form

Template:ABSTRACT PUBMED 17287215

About this Structure

2OLG is a Single protein structure of sequence from Holotrichia diomphalia. Full crystallographic information is available from OCA.

Reference

Crystal structure of the serine protease domain of prophenoloxidase activating factor-I., Piao S, Kim S, Kim JH, Park JW, Lee BL, Ha NC, J Biol Chem. 2007 Apr;282(14):10783-91. Epub 2007 Feb 7. PMID:17287215

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