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| [[Image:2ez8.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2ez8| PDB=2ez8 | SCENE= }} | | {{STRUCTURE_2ez8| PDB=2ez8 | SCENE= }} |
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| '''Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate'''
| | ===Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate=== |
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| ==Overview==
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| Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here, we report the X-ray structures of key intermediates in the oxidative decarboxylation of pyruvate, a central reaction in carbon metabolism catalyzed by the ThDP- and flavin-dependent enzyme pyruvate oxidase (POX)3 from Lactobacillus plantarum. The structures of 2-lactyl-ThDP (LThDP, 2) and its stable phosphonate analog, of 2-hydroxyethyl-ThDP (HEThDP, 3) enamine and of 2-acetyl-ThDP (AcThDP, 4; all shown bound to the enzyme's active site) provide profound insights into the chemical mechanisms and the stereochemical course of thiamin catalysis. These snapshots also suggest a mechanism for a phosphate-linked acyl transfer coupled to electron transfer in a radical reaction of pyruvate oxidase.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16680160}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16680160 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16680160}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Reaction intermediate]] | | [[Category: Reaction intermediate]] |
| [[Category: Tpp enzyme]] | | [[Category: Tpp enzyme]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:41 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:45:05 2008'' |
