2vii: Difference between revisions

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[[Image:2vii.jpg|left|200px]]
{{Seed}}
[[Image:2vii.png|left|200px]]


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{{STRUCTURE_2vii|  PDB=2vii  |  SCENE=  }}  
{{STRUCTURE_2vii|  PDB=2vii  |  SCENE=  }}  


'''PSPF1-275-MG-AMP'''
===PSPF1-275-MG-AMP===




==Overview==
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Mechanochemical proteins rely on ATP hydrolysis to establish the different functional states required for their biological output. Studying the transient functional intermediate states these proteins adopt as they progress through the ATP hydrolysis cycle is key to understanding the molecular basis of their mechanism. Many of these intermediates have been successfully 'trapped' and functionally characterised using ATP analogues. Here, we present a new nucleotide analogue, AMP-AlF(x), which traps PspF, a bacterial enhancer binding protein, in a stable complex with the sigma(54)-RNA polymerase holoenzyme. The crystal structure of AMP-AlF(x)*PspF(1-275) provides new information on protein-nucleotide interactions and suggests that the beta and gamma phosphates are more important than the alpha phosphate in terms of sensing nucleotide bound states. In addition, functional data obtained with AMP-AlF(x) establish distinct roles for the conserved catalytic AAA(+) (ATPases associated with various cellular activities) residues, suggesting that AMP-AlF(x) is a powerful new tool to study AAA(+) protein family members and, more generally, Walker motif ATPases.
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{{ABSTRACT_PUBMED_18082766}}


==About this Structure==
==About this Structure==
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[[Category: Transcription activation]]
[[Category: Transcription activation]]
[[Category: Transcription regulation,two-component regulatory system]]
[[Category: Transcription regulation,two-component regulatory system]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:52:15 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:48:20 2008''

Revision as of 10:48, 27 July 2008

File:2vii.png

Template:STRUCTURE 2vii

PSPF1-275-MG-AMP

Template:ABSTRACT PUBMED 18082766

About this Structure

2VII is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Trapping of a transcription complex using a new nucleotide analogue: AMP aluminium fluoride., Joly N, Rappas M, Buck M, Zhang X, J Mol Biol. 2008 Feb 1;375(5):1206-11. Epub 2007 Nov 22. PMID:18082766

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