1pud: Difference between revisions

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[[Image:1pud.gif|left|200px]]
{{Seed}}
[[Image:1pud.png|left|200px]]


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{{STRUCTURE_1pud|  PDB=1pud  |  SCENE=  }}  
{{STRUCTURE_1pud|  PDB=1pud  |  SCENE=  }}  


'''TRNA-GUANINE TRANSGLYCOSYLASE'''
===TRNA-GUANINE TRANSGLYCOSYLASE===




==Overview==
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tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with the queuine (.)precursor 7-aminomethyl-7-deazaguanine (preQ1). The crystal structure of TGT from Zymomonas mobilis was solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 19% at 1.85 angstrom resolution. The structure consists of an irregular (beta/alpha)8-barrel with a tightly attached C-terminal zinc-containing subdomain. The packing of the subdomain against the barrel is mediated by an alpha-helix, located close to the C-terminus, which displaces the eighth helix of the barrel. The structure of TGT in complex with preQ1 suggests a binding mode for tRNA where the phosphate backbone interacts with the zinc subdomain and the U33G34U35 sequence is recognized by the barrel. This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid.
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{{ABSTRACT_PUBMED_8654383}}


==About this Structure==
==About this Structure==
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[[Category: Transferase]]
[[Category: Transferase]]
[[Category: Trna-modifying enzyme]]
[[Category: Trna-modifying enzyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:29:37 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:07:42 2008''

Revision as of 11:07, 27 July 2008

File:1pud.png

Template:STRUCTURE 1pud

TRNA-GUANINE TRANSGLYCOSYLASE

Template:ABSTRACT PUBMED 8654383

About this Structure

1PUD is a Single protein structure of sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange., Romier C, Reuter K, Suck D, Ficner R, EMBO J. 1996 Jun 3;15(11):2850-7. PMID:8654383

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