2ltn: Difference between revisions

Line 1:

[[Image:2ltn.~~jpg~~|left|200px]]

[[Image:2ltn.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)~~'''~~

===DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)===

~~==Overview==~~

<!--

The ~~propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria~~, ~~including~~ the ~~ability~~ to ~~form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay,~~ the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. Preliminary crystallization experiments using the recombinant propeptide lectin yield crystals in space group P2(~~1)2(1)2(1) with a = 64~~.~~8 A, b = 73~~.~~8 A~~, ~~and c = 109.0 A (1 A = 0.1 nm) that diffract to 2.8-A resolution. This unit cell size~~ is ~~quite similar to~~ the ~~unit cell determined for native pea lectin, suggesting that the overall structure of the recombinant prolectin is virtually identical~~.

The line below this paragraph, {{ABSTRACT_PUBMED_2708344}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 2708344 is the PubMed ID number.

-->

==About this Structure==

Line 26:

Line 30:

[[Category: Suddath, F L.]]

[[Category: Lectin]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 09~~:31:08 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:10:10 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:2ltn.jpg|left|200px]]
+{{Seed}}
+[[Image:2ltn.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2ltn|  PDB=2ltn  |  SCENE=  }}
-'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''
+===DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)===
-==Overview==
+<!--
-The propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria, including the ability to form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. Preliminary crystallization experiments using the recombinant propeptide lectin yield crystals in space group P2(1)2(1)2(1) with a = 64.8 A, b = 73.8 A, and c = 109.0 A (1 A = 0.1 nm) that diffract to 2.8-A resolution. This unit cell size is quite similar to the unit cell determined for native pea lectin, suggesting that the overall structure of the recombinant prolectin is virtually identical.
+The line below this paragraph, {{ABSTRACT_PUBMED_2708344}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 2708344 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_2708344}}
 ==About this Structure==
@@ Line 26: / Line 30: @@
 [[Category: Suddath, F L.]]
 [[Category: Lectin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 09:31:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:10:10 2008''