1nwk: Difference between revisions

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[[Image:1nwk.gif|left|200px]]
{{Seed}}
[[Image:1nwk.png|left|200px]]


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{{STRUCTURE_1nwk|  PDB=1nwk  |  SCENE=  }}  
{{STRUCTURE_1nwk|  PDB=1nwk  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE'''
===CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE===




==Overview==
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A nucleotide-dependent conformational change regulates actin filament dynamics. Yet, the structural basis of this mechanism remains controversial. The x-ray crystal structure of tetramethylrhodamine-5-maleimide-actin with bound AMPPNP, a non-hydrolyzable ATP analog, was determined to 1.85-A resolution. A comparison of this structure to that of tetramethylrhodamine-5-maleimide-actin with bound ADP, determined previously under similar conditions, reveals how the release of the nucleotide gamma-phosphate sets in motion a sequence of events leading to a conformational change in subdomain 2. The side chain of Ser-14 in the catalytic site rotates upon Pi release, triggering the rearrangement of the loop containing the methylated His-73, referred to as the sensor loop. This in turn causes a transition in the DNase I-binding loop in subdomain 2 from a disordered loop in ATP-actin to an ordered alpha-helix in ADP-actin. Despite this conformational change, the nucleotide cleft remains closed in ADP-actin, similar to ATP-actin. An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state.
The line below this paragraph, {{ABSTRACT_PUBMED_12813032}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_12813032}}


==About this Structure==
==About this Structure==
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[[Category: Atp-state]]
[[Category: Atp-state]]
[[Category: Tetramethylrhodamine-5-maleimide]]
[[Category: Tetramethylrhodamine-5-maleimide]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:03:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:43:13 2008''

Revision as of 11:43, 27 July 2008

File:1nwk.png

Template:STRUCTURE 1nwk

CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE

Template:ABSTRACT PUBMED 12813032

About this Structure

1NWK is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics., Graceffa P, Dominguez R, J Biol Chem. 2003 Sep 5;278(36):34172-80. Epub 2003 Jun 17. PMID:12813032

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