1v7o: Difference between revisions

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[[Image:1v7o.~~jpg~~|left|200px]]

[[Image:1v7o.png|left|200px]]

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~~'''~~Alanyl-tRNA synthetase editing domain homologue protein from Pyrococcus horikoshii~~'''~~

===Alanyl-tRNA synthetase editing domain homologue protein from Pyrococcus horikoshii===

~~==Overview==~~

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~~AlaX is~~ the ~~homologue of~~ the ~~class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA~~(~~Ala)~~. ~~Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc~~. ~~Together with mutational analysis~~, ~~we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine~~ is ~~responsible for~~ the ~~discrimination of noncognate serine from cognate alanine, based on their chemical natures~~. ~~Furthermore, we confirmed that the conserved Gln~~-~~584 in alanyl~~-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).

The line below this paragraph, {{ABSTRACT_PUBMED_16087889}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16087889 is the PubMed ID number.

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==About this Structure==

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[[Category: Yao, M.]]

[[Category: Hydrolase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 12~~:11:06 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:03:46 2008''

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-[[Image:1v7o.jpg|left|200px]]
+{{Seed}}
+[[Image:1v7o.png|left|200px]]
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 {{STRUCTURE_1v7o|  PDB=1v7o  |  SCENE=  }}
-'''Alanyl-tRNA synthetase editing domain homologue protein from Pyrococcus horikoshii'''
+===Alanyl-tRNA synthetase editing domain homologue protein from Pyrococcus horikoshii===
-==Overview==
+<!--
-AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).
+The line below this paragraph, {{ABSTRACT_PUBMED_16087889}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16087889 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16087889}}
 ==About this Structure==
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 [[Category: Yao, M.]]
 [[Category: Hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:11:06 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:03:46 2008''