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| {{STRUCTURE_3bnx| PDB=3bnx | SCENE= }} | | {{STRUCTURE_3bnx| PDB=3bnx | SCENE= }} |
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| '''Crystal structure of Aristolochene synthase complexed with farnesyl diphosphate'''
| | ===Crystal structure of Aristolochene synthase complexed with farnesyl diphosphate=== |
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| ==Overview==
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| The universal sesquiterpene precursor, farnesyl diphosphate (FPP), is cyclized in an Mg(2+)-dependent reaction catalyzed by the tetrameric aristolochene synthase from Aspergillus terreus to form the bicyclic hydrocarbon aristolochene and a pyrophosphate anion (PP(i)) coproduct. The 2.1-A resolution crystal structure determined from crystals soaked with FPP reveals the binding of intact FPP to monomers A-C, and the binding of PP(i) and Mg(2+)(B) to monomer D. The 1.89-A resolution structure of the complex with 2-fluorofarnesyl diphosphate (2F-FPP) reveals 2F-FPP binding to all subunits of the tetramer, with Mg(2+)(B)accompanying the binding of this analogue only in monomer D. All monomers adopt open activesite conformations in these complexes, but slight structural changes in monomers C and D of each complex reflect the very initial stages of a conformational transition to the closed state. Finally, the 2.4-A resolution structure of the complex with 12,13-difluorofarnesyl diphosphate (DF-FPP) reveals the binding of intact DF-FPP to monomers A-C in the open conformation and the binding of PP(i), Mg(2+)(B), and Mg(2+)(C) to monomer D in a predominantly closed conformation. Taken together, these structures provide 12 independent "snapshots" of substrate or product complexes that suggest a possible sequence for metal ion binding and conformational changes required for catalysis. | | The line below this paragraph, {{ABSTRACT_PUBMED_18385128}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18385128 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18385128}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Magnesium]] | | [[Category: Magnesium]] |
| [[Category: Sesquiterpene cyclase]] | | [[Category: Sesquiterpene cyclase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:57:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:55:06 2008'' |
