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[[Image:1uix.jpg|left|200px]]
{{Seed}}
[[Image:1uix.png|left|200px]]


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{{STRUCTURE_1uix|  PDB=1uix  |  SCENE=  }}  
{{STRUCTURE_1uix|  PDB=1uix  |  SCENE=  }}  


'''Coiled-coil structure of the RhoA-binding domain in Rho-kinase'''
===Coiled-coil structure of the RhoA-binding domain in Rho-kinase===




==Overview==
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Rho-kinase is a serine/threonine protein kinase that regulates cytoskeletal events in cells. The enzyme activity of Rho-kinase is auto-inhibited in the free state but is activated through direct binding to the small GTPase Rho in the GTP-bound form. The crystal structure of the Rho-binding domain (RhoBD) of Rho-kinase has been determined at 1.8-A resolution by the multi-wavelength anomalous dispersion technique. The structure shows that RhoBD dimerizes to form a parallel coiled-coil with long consecutive alpha-helices extended to approximately 97 A and suggests that free Rho-kinase can also form a dimer through parallel self-association. At the middle region of the coiled-coil, the polypeptide chains are flexible and display loose "knobs-into-holes" packing of the side chains from both chains. RhoBD residues that have been shown to be critical for Rho-binding are spread in the positively charged C-terminal region. The parallel coiled-coil structure of our Rho-kinase RhoBD in the free form is different from the anti-parallel coiled-coil structure of RhoBD of protein kinase N when complexed with RhoA. Implications derived from these structural studies in relation to the mechanism of Rho-kinase activation will be addressed with previously reported experimental data.
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{{ABSTRACT_PUBMED_12954645}}


==About this Structure==
==About this Structure==
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[[Category: Shimizu, T.]]
[[Category: Shimizu, T.]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:17:41 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:05:48 2008''

Revision as of 13:05, 27 July 2008

File:1uix.png

Template:STRUCTURE 1uix

Coiled-coil structure of the RhoA-binding domain in Rho-kinase

Template:ABSTRACT PUBMED 12954645

About this Structure

1UIX is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Parallel coiled-coil association of the RhoA-binding domain in Rho-kinase., Shimizu T, Ihara K, Maesaki R, Amano M, Kaibuchi K, Hakoshima T, J Biol Chem. 2003 Nov 14;278(46):46046-51. Epub 2003 Sep 3. PMID:12954645

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