1s49: Difference between revisions

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[[Image:1s49.~~jpg~~|left|200px]]

[[Image:1s49.png|left|200px]]

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~~'''~~Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP~~'''~~

===Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP===

~~==Overview==~~

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The ~~bovine viral diarrhea virus (BVDV) RNA-dependent RNA polymerase can initiate RNA replication by a de novo mechanism without a primer. The structure of BVDV polymerase, determined to 2.9-A resolution~~, ~~contains a unique N-terminal domain~~, ~~in addition~~ to the ~~fingers, palm, and thumb domains common to other polymerases~~. ~~The structure of BVDV polymerase complexed with GTP~~, ~~which~~ is ~~required for de novo (primer-independent) initiation, shows that GTP binds adjacent to~~ the initiation NTP, suggesting that the GTP mimics a vestigial RNA product. Comparison of five monomers in two different crystal forms showed conformational changes in the fingertip region and in the thumb domain that may help to translocate the RNA template and product strands during elongation. The putative binding sites of previously reported BVDV inhibitors are also discussed.

The line below this paragraph, {{ABSTRACT_PUBMED_15070734}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15070734 is the PubMed ID number.

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==About this Structure==

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[[Category: Primer independent initiation]]

[[Category: Rna synthesis]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:16:34 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:17:41 2008''

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-[[Image:1s49.jpg|left|200px]]
+{{Seed}}
+[[Image:1s49.png|left|200px]]
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 {{STRUCTURE_1s49|  PDB=1s49  |  SCENE=  }}
-'''Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP'''
+===Crystal Structure of RNA-dependent RNA polymerase construct 1 (residues 71-679) from bovine viral diarrhea virus complexed with GTP===
-==Overview==
+<!--
-The bovine viral diarrhea virus (BVDV) RNA-dependent RNA polymerase can initiate RNA replication by a de novo mechanism without a primer. The structure of BVDV polymerase, determined to 2.9-A resolution, contains a unique N-terminal domain, in addition to the fingers, palm, and thumb domains common to other polymerases. The structure of BVDV polymerase complexed with GTP, which is required for de novo (primer-independent) initiation, shows that GTP binds adjacent to the initiation NTP, suggesting that the GTP mimics a vestigial RNA product. Comparison of five monomers in two different crystal forms showed conformational changes in the fingertip region and in the thumb domain that may help to translocate the RNA template and product strands during elongation. The putative binding sites of previously reported BVDV inhibitors are also discussed.
+The line below this paragraph, {{ABSTRACT_PUBMED_15070734}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15070734 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15070734}}
 ==About this Structure==
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 [[Category: Primer independent initiation]]
 [[Category: Rna synthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 08:16:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:17:41 2008''