1uj2: Difference between revisions

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[[Image:1uj2.gif|left|200px]]
{{Seed}}
[[Image:1uj2.png|left|200px]]


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{{STRUCTURE_1uj2|  PDB=1uj2  |  SCENE=  }}  
{{STRUCTURE_1uj2|  PDB=1uj2  |  SCENE=  }}  


'''Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP'''
===Crystal structure of human uridine-cytidine kinase 2 complexed with products, CMP and ADP===




==Overview==
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Uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine and cytidine and activates pharmacological ribonucleoside analogs. Here we present the crystal structures of human UCK alone and in complexes with a substrate, cytidine, a feedback inhibitor, CTP or UTP, and with phosphorylation products, CMP and ADP, respectively. Free UCK takes an alpha/beta mononucleotide binding fold and exists as a homotetramer with 222 symmetry. Upon inhibitor binding, one loop region was loosened, causing the UCK tetramer to be distorted. Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides. The first UCK structure provided the structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase, which give clues for the design of novel antitumor and antiviral ribonucleoside analogs that inhibit RNA synthesis.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase., Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F, Structure. 2004 May;12(5):751-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15130468 15130468]
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase., Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F, Structure. 2004 May;12(5):751-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15130468 15130468]
Crystallization and preliminary X-ray analysis of human uridine-cytidine kinase 2., Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1477-8. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876357 12876357]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Suzuki, N N.]]
[[Category: Suzuki, N N.]]
[[Category: Alpha/beta mononucleotide-binding hold]]
[[Category: Alpha/beta mononucleotide-binding hold]]
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