2hzc: Difference between revisions

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[[Image:2hzc.gif|left|200px]]
{{Seed}}
[[Image:2hzc.png|left|200px]]


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{{STRUCTURE_2hzc|  PDB=2hzc  |  SCENE=  }}  
{{STRUCTURE_2hzc|  PDB=2hzc  |  SCENE=  }}  


'''Crystal structure of the N-terminal RRM of the U2AF large subunit'''
===Crystal structure of the N-terminal RRM of the U2AF large subunit===




==Overview==
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The essential pre-mRNA splicing factor, U2 auxiliary factor 65KD (U2AF(65)) recognizes the polypyrimidine tract (Py-tract) consensus sequence of the pre-mRNA using two RNA recognition motifs (RRMs), the most prevalent class of eukaryotic RNA-binding domain. The Py-tracts of higher eukaryotic pre-mRNAs are often interrupted with purines, yet U2AF(65) must identify these degenerate Py-tracts for accurate pre-mRNA splicing. Previously, the structure of a U2AF(65) variant in complex with poly(U) RNA suggested that rearrangement of flexible side-chains or bound water molecules may contribute to degenerate Py-tract recognition by U2AF(65). Here, the X-ray structure of the N-terminal RRM domain of U2AF(65) (RRM1) is described at 1.47 A resolution in the absence of RNA. Notably, RNA-binding by U2AF(65) selectively stabilizes pre-existing alternative conformations of three side-chains located at the RNA interface (Arg150, Lys225, and Arg227). Additionally, a flexible loop connecting the beta2/beta3 strands undergoes a conformational change to interact with the RNA. These pre-existing alternative conformations may contribute to the ability of U2AF(65) to recognize a variety of Py-tract sequences. This rare, high-resolution view of an important member of the RRM class of RNA-binding domains highlights the role of alternative side-chain conformations in RNA recognition.
The line below this paragraph, {{ABSTRACT_PUBMED_17188295}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_17188295}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif., Thickman KR, Sickmier EA, Kielkopf CL, J Mol Biol. 2007 Feb 23;366(3):703-10. Epub 2006 Dec 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17188295 17188295]
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif., Thickman KR, Sickmier EA, Kielkopf CL, J Mol Biol. 2007 Feb 23;366(3):703-10. Epub 2006 Dec 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17188295 17188295]
Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65., Sickmier EA, Frato KE, Shen H, Paranawithana SR, Green MR, Kielkopf CL, Mol Cell. 2006 Jul 7;23(1):49-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16818232 16818232]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Rna splicing]]
[[Category: Rna splicing]]
[[Category: Rrm]]
[[Category: Rrm]]
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