1py0: Difference between revisions

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[[Image:1py0.gif|left|200px]]
{{Seed}}
[[Image:1py0.png|left|200px]]


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{{STRUCTURE_1py0|  PDB=1py0  |  SCENE=  }}  
{{STRUCTURE_1py0|  PDB=1py0  |  SCENE=  }}  


'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
===Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe===




==Overview==
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A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.
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{{ABSTRACT_PUBMED_15224334}}


==About this Structure==
==About this Structure==
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[[Category: Cupredoxin]]
[[Category: Cupredoxin]]
[[Category: Nmr probe]]
[[Category: Nmr probe]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:37:46 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:52:57 2008''

Revision as of 13:53, 27 July 2008

File:1py0.png

Template:STRUCTURE 1py0

Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe

Template:ABSTRACT PUBMED 15224334

About this Structure

1PY0 is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334

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