|
|
| Line 1: |
Line 1: |
| [[Image:2akg.gif|left|200px]] | | {{Seed}} |
| | [[Image:2akg.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_2akg| PDB=2akg | SCENE= }} | | {{STRUCTURE_2akg| PDB=2akg | SCENE= }} |
|
| |
|
| '''Thallium form of the G-Quadruplex from Oxytricha Nova, d(G4T4G4)2'''
| | ===Thallium form of the G-Quadruplex from Oxytricha Nova, d(G4T4G4)2=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Monovalent cations play an important role in many biological functions. The guanine rich sequence, d(G4T4G4), requires monovalent cations for formation of the G-quadruplex, d(G4T4G4)2. This requirement can be satisfied by thallium (Tl+), a potassium (K+) surrogate. To verify that the structure of d(G4T4G4)2 in the presence of Tl+ is similar to the K+-form of the G-quadruplex, the solution structure of the Tl+-form of d(G4T4G4)2 was determined. The 10 lowest energy structures have an all atom RMSD of 0.76 +/- 0.16 A. Comparison of this structure to the identical G-quadruplex formed in the presence of K+ validates the isomorphous nature of Tl+ and K+. Using a 1H-205Tl spin-echo difference experiment we show that, in the Tl+-form of d(G4T4G4)2, small scalar couplings (<1 Hz) exist between 205Tl and protons in the G-quadruplex. These data comprise the first 1H-205Tl scalar couplings observed in a biological system and have the potential to provide important constraints for structure determination. These experiments can be applied to any system in which the substituted Tl+ cations are in slow exchange with the bulk ions in solution.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16305263}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16305263 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16305263}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AKG OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 26: |
Line 30: |
| [[Category: G-quadruplex]] | | [[Category: G-quadruplex]] |
| [[Category: Thallium]] | | [[Category: Thallium]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:09:29 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:54:36 2008'' |
