1t5t: Difference between revisions

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[[Image:1t5t.~~gif~~|left|200px]]

[[Image:1t5t.png|left|200px]]

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~~'''~~Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form~~'''~~

===Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form===

~~==Overview==~~

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~~A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has~~ to ~~be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco~~(~~endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA)~~ at 2.~~6 and 2~~.~~9 angstrom resolution in complex with (i~~) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, ~~thereby preventing backflow before Ca2+~~ is ~~released on the other side of~~ the ~~membrane~~.

The line below this paragraph, {{ABSTRACT_PUBMED_15192230}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15192230 is the PubMed ID number.

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==About this Structure==

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[[Category: Phosphorylation]]

[[Category: Transition state]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 09~~:33:46 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:54:57 2008''

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-[[Image:1t5t.gif|left|200px]]
+{{Seed}}
+[[Image:1t5t.png|left|200px]]
 <!--
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 {{STRUCTURE_1t5t|  PDB=1t5t  |  SCENE=  }}
-'''Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form'''
+===Structure of the (SR)Ca2+-ATPase Ca2-E1-ADP:AlF4- form===
-==Overview==
+<!--
-A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+-adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (beta-gamma methylene)-triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1 approximately P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane.
+The line below this paragraph, {{ABSTRACT_PUBMED_15192230}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15192230 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15192230}}
 ==About this Structure==
@@ Line 32: / Line 36: @@
 [[Category: Phosphorylation]]
 [[Category: Transition state]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 09:33:46 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:54:57 2008''