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| {{STRUCTURE_2f8u| PDB=2f8u | SCENE= }} | | {{STRUCTURE_2f8u| PDB=2f8u | SCENE= }} |
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| '''Solution structure of G-quadruplex formed in the human Bcl-2 promoter'''
| | ===Solution structure of G-quadruplex formed in the human Bcl-2 promoter=== |
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| ==Overview==
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| BCL2 protein functions as an inhibitor of cell apoptosis and has been found to be aberrantly expressed in a wide range of human diseases. A highly GC-rich region upstream of the P1 promoter plays an important role in the transcriptional regulation of BCL2. Here we report the NMR solution structure of the major intramolecular G-quadruplex formed on the G-rich strand of this region in K+ solution. This well-defined mixed parallel/antiparallel-stranded G-quadruplex structure contains three G-tetrads of mixed G-arrangements, which are connected with two lateral loops and one side loop, and four grooves of different widths. The three loops interact with the core G-tetrads in a specific way that defines and stabilizes the overall G-quadruplex structure. The loop conformations are in accord with the experimental mutation and footprinting data. The first 3-nt loop adopts a lateral loop conformation and appears to determine the overall folding of the BCL2 G-quadruplex. The third 1-nt double-chain-reversal loop defines another example of a stable parallel-stranded structural motif using the G3NG3 sequence. Significantly, the distinct major BCL2 promoter G-quadruplex structure suggests that it can be specifically involved in gene modulation and can be an attractive target for pathway-specific drug design.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16998187}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16998187 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16998187}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8U OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: G-quadruplex]] | | [[Category: G-quadruplex]] |
| [[Category: Nmr]] | | [[Category: Nmr]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:36:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:59:00 2008'' |
