1mw8: Difference between revisions

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[[Image:1mw8.gif|left|200px]]
{{Seed}}
[[Image:1mw8.png|left|200px]]


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{{STRUCTURE_1mw8|  PDB=1mw8  |  SCENE=  }}  
{{STRUCTURE_1mw8|  PDB=1mw8  |  SCENE=  }}  


'''Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3''''
===Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'===




==Overview==
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In order to gain insights into the mechaism of ssDNA binding and recognition by Escherichia coli DNA topoisomerase I, the structure of the 67 kDa N-terminal fragment of topoisomerase I was solved in complex with ssDNA. The structure reveals a new conformational stage in the multistep catalytic cycle of type IA topoisomerases. In the structure, the ssDNA binding groove leading to the active site is occupied, but the active site is not fully formed. Large conformational changes are not seen; instead, a single helix parallel to the ssDNA binding groove shifts to clamp the ssDNA. The structure helps clarify the temporal sequence of conformational events, starting from an initial empty enzyme and proceeding to a ssDNA-occupied and catalytically competent active site.
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{{ABSTRACT_PUBMED_14604525}}


==About this Structure==
==About this Structure==
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[[Category: Dna topoisomerase]]
[[Category: Dna topoisomerase]]
[[Category: Toprim domain]]
[[Category: Toprim domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:47:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:31:18 2008''

Revision as of 14:31, 27 July 2008

File:1mw8.png

Template:STRUCTURE 1mw8

Crystal Structure of a Complex between H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I and 5'-ACTTCGGGATG-3'

Template:ABSTRACT PUBMED 14604525

About this Structure

1MW8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA., Perry K, Mondragon A, Structure. 2003 Nov;11(11):1349-58. PMID:14604525

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