1mqv: Difference between revisions

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[[Image:1mqv.gif|left|200px]]
{{Seed}}
[[Image:1mqv.png|left|200px]]


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{{STRUCTURE_1mqv|  PDB=1mqv  |  SCENE=  }}  
{{STRUCTURE_1mqv|  PDB=1mqv  |  SCENE=  }}  


'''Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli'''
===Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli===




==Overview==
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We employed fluorescence energy-transfer probes to investigate the polypeptide dynamics accompanying cytochrome c' folding. Analysis of fluorescence energy-transfer kinetics from wild-type Trp-72 or Trp-32 in a crystallographically characterized (1.78 A) Q1A/F32W/W72F mutant shows that there is structural heterogeneity in denatured cytochrome c'. Even at guanidine hydrochloride concentrations well beyond the unfolding transition, a substantial fraction of the polypeptides ( approximately 50%) adopts compact conformations (tryptophan-to-heme distance, approximately 25 A) in both pseudo-wild-type (Q1A) and mutant proteins. A burst phase (&lt; or =5 ms) is revealed when stopped flow-triggered refolding is probed by tryptophan intensity: measurements on the Q1A protein show that approximately 75% of the Trp-72 fluorescence (83% for Trp-32) is quenched within the mixing deadtime, suggesting that most of the polypeptides have collapsed.
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==About this Structure==
==About this Structure==
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[[Category: Winkler, J R.]]
[[Category: Winkler, J R.]]
[[Category: Four-helix bundle]]
[[Category: Four-helix bundle]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:36:44 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:33:37 2008''

Revision as of 14:33, 27 July 2008

File:1mqv.png

Template:STRUCTURE 1mqv

Crystal Structure of the Q1A/F32W/W72F mutant of Rhodopseudomonas palustris cytochrome c' (prime) expressed in E. coli

Template:ABSTRACT PUBMED 12407175

About this Structure

1MQV is a Single protein structure of sequence from Rhodopseudomonas palustris. Full crystallographic information is available from OCA.

Reference

Structural features of cytochrome c' folding intermediates revealed by fluorescence energy-transfer kinetics., Lee JC, Engman KC, Tezcan FA, Gray HB, Winkler JR, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14778-82. Epub 2002 Oct 29. PMID:12407175

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