2yz1: Difference between revisions

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[[Image:2yz1.jpg|left|200px]]
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[[Image:2yz1.png|left|200px]]


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{{STRUCTURE_2yz1|  PDB=2yz1  |  SCENE=  }}  
{{STRUCTURE_2yz1|  PDB=2yz1  |  SCENE=  }}  


'''Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha'''
===Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha===




==Overview==
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SRC homology 2 domain-containing protein tyrosine phosphatase substrate 1 (SHPS-1 or SIRP alpha/BIT) is an immunoglobulin (Ig) superfamily transmembrane receptor and a member of the signal regulatory protein (SIRP) family involved in cell-cell interaction. SHPS-1 binds to its ligand CD47 to relay an inhibitory signal for cellular responses, whereas SIRPbeta, an activating member of the same family, does not bind to CD47 despite sharing a highly homologous ligand-binding domain with SHPS-1. To address the molecular basis for specific CD47 recognition by SHPS-1, we present the crystal structure of the ligand-binding domain of murine SHPS-1 (mSHPS-1). Folding topology revealed that mSHPS-1 adopts an I2-set Ig fold, but its overall structure resembles IgV domains of antigen receptors, although it has an extended loop structure (C'E loop), which forms a dimer interface in the crystal. Site-directed mutagenesis studies of mSHPS-1 identified critical residues for CD47 binding including sites in the C'E loop and regions corresponding to complementarity-determining regions of antigen receptors. The structural and functional features of mSHPS-1 are consistent with the human SHPS-1 structure except that human SHPS-1 has an additional beta-strand D. These results suggest that the variable complementarity-determining region-like loop structures in the binding surface of SHPS-1 are generally required for ligand recognition in a manner similar to that of antigen receptors, which may explain the diverse ligand-binding specificities of SIRP family receptors.
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{{ABSTRACT_PUBMED_18045614}}


==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transmembrane]]
[[Category: Transmembrane]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 19:43:11 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:36:19 2008''

Revision as of 14:36, 27 July 2008

File:2yz1.png

Template:STRUCTURE 2yz1

Crystal structure of the ligand-binding domain of murine SHPS-1/SIRP alpha

Template:ABSTRACT PUBMED 18045614

About this Structure

2YZ1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural insight into the specific interaction between murine SHPS-1/SIRP alpha and its ligand CD47., Nakaishi A, Hirose M, Yoshimura M, Oneyama C, Saito K, Kuki N, Matsuda M, Honma N, Ohnishi H, Matozaki T, Okada M, Nakagawa A, J Mol Biol. 2008 Jan 18;375(3):650-60. Epub 2007 Nov 7. PMID:18045614

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