1mwj: Difference between revisions

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[[Image:1mwj.gif|left|200px]]
{{Seed}}
[[Image:1mwj.png|left|200px]]


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{{STRUCTURE_1mwj|  PDB=1mwj  |  SCENE=  }}  
{{STRUCTURE_1mwj|  PDB=1mwj  |  SCENE=  }}  


'''Crystal Structure of a MUG-DNA pseudo substrate complex'''
===Crystal Structure of a MUG-DNA pseudo substrate complex===




==Overview==
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The bacterial mismatch-specific uracil-DNA glycosylase (MUG) and eukaryotic thymine-DNA glycosylase (TDG) enzymes form a homologous family of DNA glycosylases that initiate base-excision repair of G:U/T mismatches. Despite low sequence homology, the MUG/TDG enzymes are structurally related to the uracil-DNA glycosylase enzymes, but have a very different mechanism for substrate recognition. We have now determined the crystal structure of the Escherichia coli MUG enzyme complexed with an oligonucleotide containing a non-hydrolysable deoxyuridine analogue mismatched with guanine, providing the first structure of an intact substrate-nucleotide productively bound to a hydrolytic DNA glycosylase. The structure of this complex explains the preference for G:U over G:T mispairs, and reveals an essentially non-specific pyrimidine-binding pocket that allows MUG/TDG enzymes to excise the alkylated base, 3, N(4)-ethenocytosine. Together with structures for the free enzyme and for an abasic-DNA product complex, the MUG-substrate analogue complex reveals the conformational changes accompanying the catalytic cycle of substrate binding, base excision and product release.
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==About this Structure==
==About this Structure==
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[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
[[Category: Uracil recognition.]]
[[Category: Uracil recognition.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:48:21 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:51:45 2008''

Revision as of 14:51, 27 July 2008

File:1mwj.png

Template:STRUCTURE 1mwj

Crystal Structure of a MUG-DNA pseudo substrate complex

Template:ABSTRACT PUBMED 10581234

About this Structure

1MWJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a thwarted mismatch glycosylase DNA repair complex., Barrett TE, Scharer OD, Savva R, Brown T, Jiricny J, Verdine GL, Pearl LH, EMBO J. 1999 Dec 1;18(23):6599-609. PMID:10581234

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