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| [[Image:1s23.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1s23| PDB=1s23 | SCENE= }} | | {{STRUCTURE_1s23| PDB=1s23 | SCENE= }} |
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| '''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''
| | ===Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG=== |
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| ==Overview==
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| The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures. | | The line below this paragraph, {{ABSTRACT_PUBMED_15039556}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15039556 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15039556}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Deoxyoligonucleotide]] | | [[Category: Deoxyoligonucleotide]] |
| [[Category: Variability]] | | [[Category: Variability]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:12:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:01:12 2008'' |
