2jtq: Difference between revisions

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[[Image:2jtq.jpg|left|200px]]
{{Seed}}
[[Image:2jtq.png|left|200px]]


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{{STRUCTURE_2jtq|  PDB=2jtq  |  SCENE=  }}  
{{STRUCTURE_2jtq|  PDB=2jtq  |  SCENE=  }}  


'''Rhodanese from E.coli'''
===Rhodanese from E.coli===




==Overview==
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Rhodanese catalyzes the sulfur-transfer reaction that transfers sulfur from thiosulfate to cyanide by a double-displacement mechanism, in which an active cysteine residue plays a central role. Previous studies indicated that the phage-shock protein E (PspE) from Escherichia coli is a rhodanese composed of a single active domain and is the only accessible rhodanese among the three single-domain rhodaneses in E. coli. To understand the catalytic mechanism of rhodanese at the molecular level, we determined the solution structures of the sulfur-free and persulfide-intermediate forms of PspE by nuclear magnetic resonance (NMR) spectroscopy and identified the active site by NMR titration experiments. To obtain further insights into the catalytic mechanism, we studied backbone dynamics by NMR relaxation experiments. Our results demonstrated that the overall structures in both sulfur-free and persulfide-intermediate forms are highly similar, suggesting that no significant conformational changes occurred during the catalytic reaction. However, the backbone dynamics revealed that the motional properties of PspE in its sulfur-free form are different from the persulfide-intermediate state. The conformational exchanges are largely enhanced in the persulfide-intermediate form of PspE, especially around the active site. The present structural and biochemical studies in combination with backbone dynamics provide further insights in understanding the catalytic mechanism of rhodanese.
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{{ABSTRACT_PUBMED_18355042}}


==About this Structure==
==About this Structure==
2JTQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTQ OCA].  
2JTQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTQ OCA].  


==Reference==
==Reference==
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[[Category: Stress response]]
[[Category: Stress response]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:02:10 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:03:38 2008''

Revision as of 15:03, 27 July 2008

File:2jtq.png

Template:STRUCTURE 2jtq

Rhodanese from E.coli

Template:ABSTRACT PUBMED 18355042

About this Structure

2JTQ is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structures and backbone dynamics of Escherichia coli rhodanese PspE in its sulfur-free and persulfide-intermediate forms: implications for the catalytic mechanism of rhodanese., Li H, Yang F, Kang X, Xia B, Jin C, Biochemistry. 2008 Apr 15;47(15):4377-85. Epub 2008 Mar 21. PMID:18355042

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