1pdc: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1pdc.gif|left|200px]]
{{Seed}}
[[Image:1pdc.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pdc|  PDB=1pdc  |  SCENE=  }}  
{{STRUCTURE_1pdc|  PDB=1pdc  |  SCENE=  }}  


'''REFINED SOLUTION STRUCTURE AND LIGAND-BINDING PROPERTIES OF PDC-109 DOMAIN B. A COLLAGEN-BINDING TYPE II DOMAIN'''
===REFINED SOLUTION STRUCTURE AND LIGAND-BINDING PROPERTIES OF PDC-109 DOMAIN B. A COLLAGEN-BINDING TYPE II DOMAIN===




==Overview==
<!--
We have determined, via 1H-n.m.r., the solution conformation of the collagen-binding b-domain of the bovine seminal fluid protein PDC-109 (PDC-109/b). The structure determination is based on 341 interproton distance estimates and 42 dihedral angle estimates: a set of 24 initial structures were computed; 12 using the variable target function program DIANA, and 12 using the metric matrix program DISGEO. These structures were optimized by restrained energy minimization and dynamic simulated annealing using the CHARMM and X-PLOR programs. The average pairwise root-mean-square difference (r.m.s.d) between the optimized DIANA (DISGEO) structures is 0.71 A (0.82 A) for the backbone atoms, and 1.73 A (2.03 A) for all atoms. Both sets of structures exhibit the same global fold, secondary structure and placement of most non-polar side-chains. Two central antiparallel beta-sheets, which lie roughly perpendicular to each other, and two irregular loops support a large, partially exposed, hydrophobic surface that defines a putative binding site. A test of a hybrid relaxation matrix-based distance refinement protocol (MIDGE program) was performed using a normalized 250 millisecond NOESY spectrum. The resulting distances were input to the molecular mechanics/dynamics procedures mentioned above in order to optimize the DIANA structures. Our results indicate that relaxation matrix refinement of distances is most useful when used conservatively for identifying underestimated distance constraints. 1H-n.m.r. monitored ligand titration experiments revealed definite, albeit weak, binding interactions for phenethylamine and leucine analogs (Ka less than or equal to 25 M-1). Residues perturbed by ligand binding include Tyr7, Trp26, Tyr33, Asp34 and Trp39. These results suggest that PDC-109/b may recognize specific leucine and/or isoleucine-containing sequences within collagen.
The line below this paragraph, {{ABSTRACT_PUBMED_1731074}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 1731074 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_1731074}}


==About this Structure==
==About this Structure==
1PDC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDC OCA].  
1PDC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDC OCA].  


==Reference==
==Reference==
Line 26: Line 30:
[[Category: Patthy, L.]]
[[Category: Patthy, L.]]
[[Category: Collagen-binding type ii domain]]
[[Category: Collagen-binding type ii domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 04:57:46 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:59:15 2008''

Revision as of 15:59, 27 July 2008

File:1pdc.png

Template:STRUCTURE 1pdc

REFINED SOLUTION STRUCTURE AND LIGAND-BINDING PROPERTIES OF PDC-109 DOMAIN B. A COLLAGEN-BINDING TYPE II DOMAIN

Template:ABSTRACT PUBMED 1731074

About this Structure

1PDC is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

Refined solution structure and ligand-binding properties of PDC-109 domain b. A collagen-binding type II domain., Constantine KL, Madrid M, Banyai L, Trexler M, Patthy L, Llinas M, J Mol Biol. 1992 Jan 5;223(1):281-98. PMID:1731074

Page seeded by OCA on Sun Jul 27 18:59:15 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1pdc&oldid=661945"