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| [[Image:1r52.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1r52| PDB=1r52 | SCENE= }} | | {{STRUCTURE_1r52| PDB=1r52 | SCENE= }} |
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| '''Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae'''
| | ===Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae=== |
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| ==Overview==
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| Chorismate synthase (EC 4.2.3.5), the seventh enzyme in the shikimate pathway, catalyzes the transformation of 5-enolpyruvylshikimate 3-phosphate (EPSP) to chorismate, which is the last common precursor in the biosynthesis of numerous aromatic compounds in bacteria, fungi, and plants. The chorismate synthase reaction involves a 1,4-trans-elimination of phosphoric acid from EPSP and has an absolute requirement for reduced FMN as a cofactor. We have determined the three-dimensional x-ray structure of the yeast chorismate synthase from selenomethionine-labeled crystals at 2.2-A resolution. The structure shows a novel betaalphabetaalpha fold consisting of an alternate tight packing of two alpha-helical and two beta-sheet layers, showing no resemblance to any documented protein structure. The molecule is arranged as a tight tetramer with D2 symmetry, in accordance with its quaternary structure in solution. Electron density is missing for 23% of the amino acids, spread over sequence regions that in the three-dimensional structure converge on the surface of the protein. Many totally conserved residues are contained within these regions, and they probably form a structured but mobile domain that closes over a cleft upon substrate binding and catalysis. This hypothesis is supported by previously published spectroscopic measurements implying that the enzyme undergoes considerable structural changes upon binding of both FMN and EPSP.
| | The line below this paragraph, {{ABSTRACT_PUBMED_14573601}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14573601 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14573601}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Tilbeurgh, H van.]] | | [[Category: Tilbeurgh, H van.]] |
| [[Category: Two layers alpha-beta]] | | [[Category: Two layers alpha-beta]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:05:11 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:36:43 2008'' |
