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| [[Image:1ue3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1ue3| PDB=1ue3 | SCENE= }} | | {{STRUCTURE_1ue3| PDB=1ue3 | SCENE= }} |
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| '''Crystal structure of d(GCGAAAGC) containing hexaamminecobalt'''
| | ===Crystal structure of d(GCGAAAGC) containing hexaamminecobalt=== |
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| ==Overview==
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| A DNA fragment d(GCGAAAGC), postulated to adopt a stable mini-hairpin structure on the basis of its extraordinary properties, has been X-ray analyzed. Two octamers related by a crystallographic twofold symmetry are aligned in an antiparallel fashion and associate to form a duplex, which is maintained by two Watson-Crick G.C base pairs and a subsequent sheared G.A pair at both ends. The central two A residues are free from base-pair formation. The corresponding base moieties of the two strands are intercalated and stacked on each other, forming a long column of G(1)-C(2)-G(3)-A(4)-A(5)(*)-A(5)-A(4)(*)-G(3)(*)-C(2)(*)-G(1)(*) (asterisks indicate the counter-strand). The Watson-Crick and major-groove sites of the four stacked adenine bases are exposed to the solvent region, suggesting a functional role. Since this structural motif is similar to those found in the nonamers d(G(Br)CGAAAGCT) and d(G(I)CGAAAGCT), the base-intercalated duplex may be a stable form of the specific sequence. Electrophoresis results suggest that the octamer has two states, monomeric and dimeric, in solution depending on the Mg(2+) concentration. The present duplex is preferred under the crystallization conditions, which correspond to physiologically allowed conditions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_14684897}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14684897 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14684897}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sheared g:a pair]] | | [[Category: Sheared g:a pair]] |
| [[Category: Zipper-like duplex]] | | [[Category: Zipper-like duplex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:05:32 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:49:05 2008'' |
